Measurement
Part:BBa_K1461236
Designed by: Takefumi Yoshikawa Group: iGEM14_UT-Tokyo (2014-09-29)
resettable counter device using ecf11 (w/o crRBS, taRNA)
This part is a basic device in our σ-Re Counter project without riboregulator system. Once arabinoce is added, the cell generates ecf11, and ecf11 positive feedback is activated. The chassis continue to generate ecf11, which activates the expression of GFP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3495
Illegal BamHI site found at 65 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3228
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1660
[edit]
Categories
Parameters
None |