Measurement

Part:BBa_K1461236

Designed by: Takefumi Yoshikawa   Group: iGEM14_UT-Tokyo   (2014-09-29)


resettable counter device using ecf11 (w/o crRBS, taRNA)

This part is a basic device in our σ-Re Counter project without riboregulator system. Once arabinoce is added, the cell generates ecf11, and ecf11 positive feedback is activated. The chassis continue to generate ecf11, which activates the expression of GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3495
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3228
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1660


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Categories
Parameters
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